00:29:23 Christina Johnson: So excited to be here! 00:29:56 Lorrie Irwin: morning patrick 00:30:04 Rashmi Shivni: Looking forward to these talks! 00:31:07 Lorrie Irwin: so thankful these are Zoom 00:31:09 Ulf E Andersson: Looking forward to a great workshop! 00:36:42 Uzma Manzoor: good to join 00:43:14 Cecilia Mourey: 😂 00:44:51 Christina Johnson: So happy you're here, Dr. Massa!!! 00:45:18 Lorrie Irwin: Welcome Gioia Gladd your safe from hurricanes 00:45:25 Stephen: Feel better soon, Gioia! 00:58:00 Christina Johnson: I love how happy these Astronauts are when they work with the plants! 01:01:52 Patrick Grove: If you have a question for Gioia, feel free to drop it in the Q&A box! 01:03:00 Ben Sikes: Between 03 and 04, were there any other plants put in there (e.g., crop rotation)? Is there knowledge transfer from agricultural about the buildup of species-specific microbes with repeated cultivation? 01:03:11 Ben Sikes: Whoops. Will put it there. 01:07:28 Christina Johnson: So exciting that PH-07 will have Red Romaine lettuce! Thanks for giving us a sneak-peak! 01:08:37 Don: I'm excited that it will be looking at the failure state and plant stresses. Red Romaine is a bonus! 01:08:41 Melanie Correll: Exciting to do a water treatment in space 🙂 Its about time. 01:09:46 Daniel Tompkins: tebuconazole is used to treat fusarium in wooden stakes to reuse them for tomato production in Florida. UF publications should be available on EDIS. 01:18:10 Cecilia Mourey: Thank you Dr. Massa! Glad you’re safe and hope you feel better soon. 01:18:16 Ben Sikes: 👏 01:18:34 Rashmi Shivni: 👏 Thank you, Dr. Massa! 01:19:09 Àlex Calvo: Fascinating, thanks Dr. Massa! 01:21:28 Kevin: Thank you 01:38:12 Christina Johnson: AstroSkin is enabling so much research - absolutely fascinating! Thanks for sharing about this. 01:38:44 Christina Johnson: https://hexoskin.com/pages/astroskin-vital-signs-monitoring-platform-for-advanced-research 01:45:35 Cecilia Mourey: Inspirational! 01:46:26 Christina Johnson: https://nature.com/immersive/d42859-024-00009-8/index.html 01:47:03 Christina Johnson: https://osdr.nasa.gov/bio/repo 01:48:38 Rashmi Shivni: Amazing talk, Yvette! 01:49:23 Yvette: A few resources: For Bioastronautics training, research collaborations, and courses: International Institute for Astronautical Sciences https://astronauticsinstitute.org/ 01:49:35 Yvette: Preparing a payload for space: Understand your Test Readiness Level (TRL). Test and evaluate to get it past that TRL 6 level, and ideally TRL 8 (flight qualified). You can do this with your research or university institute. You can also work with peers in the IIAS. TRL 9 the technology has already been proven to work during a flight mission in space. Launch provider payload and science teams will guide you through the process. 01:49:47 Yvette: Testing Experiments in parabolic flight: The National Research Council of Canada hosts parabolic flight campaigns. You can go through the IIAS annual campaigns or reach out to NRC https://nrc.canada.ca/en. 01:49:55 Yvette: For getting your payloads in space: Sign up for all launch provider newsletters and email updates. Respond to calls for proposals that are issued. 01:50:02 Yvette: Get research certifications: Collaborative Institutional Training Initiative (CITI) offers online courses for human subjects and General Data Protection Regulation (GDPR) research. https://about.citiprogram.org/. 01:54:00 Patrick Grove: Great tips, Yvette, thanks! 01:57:37 Melanie Correll: I now need to by “old” virgin olive oil 😆 for antioxidant properties 01:57:47 Melanie Correll: buy 02:03:51 Ben Sikes: 👏 02:04:23 Rashmi Shivni: 👏 Thank you Celine, great talk! 02:04:43 Melanie Correll: 👏 02:08:36 Christina Johnson: This is a really fascinating topic! Thank you!! 02:09:19 luizzy veras: Merci beaucoup Céline 02:25:56 Ivonne Rodriguez Ramirez: Hello everyone, it’s a pleasure to share this virtual space with you all, and learn about these fascinating topics. I’d like to take these few minutes to ask for your advice. I am a young scientist from Costa Rica (email: ivonne.beatriz.rr97@gmail.com), I just finished my bachelor's degree in molecular biology and biotechnology, and I work with deep-sea fungi, analyzing their enzymatic capacities and biotechnological potential. I want to enter the field of astrobiology, but I don’t know where to start. I would appreciate it if you could share resources or advice on how to do so :) 02:27:38 Morgan: Hi Ivonne! What an interesting research topic! I'd be happy to chat with you, I will reach out via email. 02:29:53 Ivonne Rodriguez Ramirez: Hi! Morgan! I would love to chat! Thanks 02:30:06 Hermes Hernan Bolivar Torres: Hello Ivonne and Morgan!! I am form Colombia and I am working in astrobiology some years ago, I am really interesting to keep in touch with you!! 02:35:46 Ivonne Rodriguez Ramirez: Hola Hermes! please do 02:39:33 LukeFountain: Hi Sharon, I also work on plant nitrogen uptake in space, and would be interested in hearing your thoughts on the potential for co-cultivation of N-fixing crops like legumes with those that cannot fix N in the space environment? 02:44:34 LukeFountain: Thanks! Legumes can provide beneficial nutrition for astronauts too so wondering if co-cultivation could work in tandem with your approach to maximise productivity. Let’s connect and stay in touch! 02:48:05 Melanie Correll: Very exciting work on microbiomes 🙂 and endophytes, we don’t know much yet do we - future genetic engineering studies! 02:54:25 LukeFountain: If pH is the driver, and the increase is due to nitrate uptake, have you tried using a combination of ammonium and nitrate as the N source to balance the pH effects? Or is there some other driver causing pH shifts too? 02:58:06 Daniel Tompkins: bicarbonates? 03:02:20 LukeFountain: Thanks Frieda, it’s something I’ll touch on briefly tomorrow but I’m considering it as an approach for maintaining solution pH in hydroponics 03:02:37 Jane: Could an abiotic buffer help? Some kind of mineral? 03:05:10 Daniel Tompkins: to isolate maybe add chitinases 03:07:20 Jane: Fungi! I believe some degrade chitin and live Daphnia should be able to cope. 03:08:29 Christina Johnson: https://fish.uw.edu/2018/12/centennial-story-55-frieda-b-taub-staff-faculty-emerita-1959-present/ 03:08:55 Christina Johnson: https://friedaaub.academia.edu/ 03:10:07 Christina Johnson: https://www.cnosat10.com/luis-guzman 03:10:37 Daniel Tompkins: for nematode control look into chitin focused approaches. there have been efforts to add seafood waste to farm fields to build chitenases to control soil plant pest nematodes. 03:15:34 Daniel Tompkins: molecular model of a plastic polymer as a cloud bio sculpture. 03:22:30 Ted Tagami @ Magnitude.io: Fabulous! 03:22:41 Andrew Planet: I really enjoyed that one too 03:22:44 Rashmi Shivni: Lovely work! 03:24:02 Andrew Planet: So does the same species of diatom have specifically morphologically different phenotypes? 03:26:55 Andrew Planet: On thanks, I asked that because I thought they might be polyphenism of the same genome 03:28:34 Andrew Planet: So different morphology might mean inherently different physiology because of a different environment feed 03:34:04 Christina Johnson: HACCP! https://spinoff.nasa.gov/moon-landing-food-safety 03:40:40 Andrew Planet: I was getting hungry, yes very advanced! 03:44:23 Daniel Tompkins: other than nitrogen use efficiency, how does N look for this approach? I don't think this paper addresses it but haven't read it yet.https://doi.org/10.1038/s41526-024-00428-x 03:45:01 Daniel Tompkins: as in source, nitrate ammonia 03:45:02 Ted Tagami @ Magnitude.io: what are your return protocols? Are they returned in cold stowage? 03:46:21 Daniel Tompkins: membrane based yeh, absolutely it is complicated 03:46:57 Kevin: For this system on the moon, how do you deal with nitrogen in situ resource utilization? As I understand there is virtually no nitrogen or hydrogen on the moon 03:47:14 Daniel Tompkins: system level approach with Daniel yeh and membrane based separation. 03:48:12 Daniel Tompkins: Daniel Yeh is at KSC 03:49:23 Andrew Planet: See you all tomorrow. Thanks! 03:49:50 LukeFountain: Daniel Yeh is at USF but his AnMBR system is being tested at KSC 03:49:59 Andrew Planet: I badly organised my time and its clashed with another event 03:52:31 Patrick Grove: No worries, we'll catch ya tomorrow! 03:54:06 Andrew Planet: Learnt my lesson, next year I hope to plan time and prep better. Relocated overseas so adapting. All this is very much high quality advanced 03:55:05 Patrick Grove: Context regarding Daniel Yeh's Anaerobic Membrane Bioreactor - it's part of a fecal waste recycling system they're calling BioWATER or something. It's a series of bioreactors and membranes converting solid waste into nutrients/biomass for downstream use in hydroponics/aquaponics for closing the waste/food loops 04:58:08 Colin: Hi Christina! Hi Frieda! 05:21:56 Colin: TY!! 05:22:44 Christina Johnson: what a great example of using these technologies on Earth and thinking ahead to space applications 05:23:05 Colin: TY Christina!! 05:23:16 Colin: Trying to stay on my craft, haha. 05:24:09 Yvette: No cannot hear it 05:24:19 Ivonne Rodriguez Ramirez: i can't hear 05:25:01 Yvette: Voila! 05:26:59 Patrick Grove: drcaswell@spaceportaustralia.com.au 05:54:35 Lorrie Irwin: absolutely fascinating discussion very impactful for all Arctic people 06:00:15 Christina Johnson: I really appreciated this lecture and I am glad she gave us her contact email 06:16:47 Christina Johnson: We're glad you're here, Oscar! Especially in the face of the general devastation that has recently hit around you in Florida. 06:17:27 Colin: 👏 06:25:13 Benjamin Greaves: Is the “NASA - Bioregenerative Life Support Testing” partnership timeline in this presentation the most up to date? I would be interested in seeing the university partnerships all the way up until today 06:25:16 Christina Johnson: Great, simple graphics, Oscar! 06:25:36 Benjamin Greaves: Thanks, Oscar! 06:25:53 Kevin: Hi Oscar, I have a question. My Question: In one of your slides you showed a figure where nutrients was being removed from regolith and being supllied into the system for growing crops. Currently what research is being done in that area and what are the current ideas? 06:27:29 Frieda: A major contribution of the space program to earth's ecosystem would be using waste products for food production, instead of our current system of pushing our wastes into (minimally processed) sewage into rivers. Disease transmission and "Yuk factor" are problems. 06:29:06 Jane: Thermophilic composting is highly effective at killing pathogens. 06:30:18 Oscar: please send me questions to oscar.a.monje@nasa.gov... Its hard forme to type answers on phone 06:35:33 Frieda: Thank you Jane, but we flush human wastes into sewage treatment plants (well 50% of habitations are connected); the use of chlorine to kill microbes results in carcinogenic end products, so we depend on dilution into the receiving river??? 06:37:43 Christina Johnson: "Low throughput" haha 06:40:24 Jane: Awesome talk! Any publications we could read? 06:40:56 Jane: Or if he has any publications. 06:41:43 Colin: 👏 06:44:05 Christina Johnson: great suite of presentations today! 06:45:10 Don: I don't have any about the MEC, at the moment. shooting for the end of the year to have the GSUA and basic evaluation out. I do have a nice review on sustainable tech and reporting in cea though! https://doi.org/10.1007/s10669-024-09964-z 07:04:52 Don: Wait, did he say he has been living in that bus since sometime last year? 07:05:22 Don: Nice. I'm going to have to look that up. 07:05:32 Christina Johnson: I hope his Internet picks up again soon! 07:05:54 Colin: I toured the bus (MASH) two nights ago, yup, he’s living in it 07:06:07 Colin: We were both out at MDRS on the refit crew 07:09:33 Christina Johnson: looks like eggs from spirulina-fed quail have positive benefits for human health! related article. so neat! https://pubmed.ncbi.nlm.nih.gov/31353724/ 07:10:07 Patrick Grove: that's pretty neat, thanks for sharing! 07:16:15 Don: I would pick hand feeding fish over automated too. There is something satisfying about it. 07:16:45 Kevin: Hi Donald, this is awesome! what percent of your food comes from the MASH? 07:17:17 Frieda: Interesting! You mentioned fly larvae, what about the adult flys? 07:21:45 Kevin: How did you develop the idea for this project? What background helped you have the skills to execute this from a science and actually building standpoint 07:22:06 Benjamin Greaves: How is best to contact you to learn more about the MASH? 00:30:22 Kevin: So basically the Martian ! 00:30:49 Patrick Grove: www.earthseed.space 00:42:44 Christina Johnson: Magenta Jars! that looks like that setup could easily be adapted for use in the Veggie hardware on the ISS. 00:45:11 Colin: Great presentation! 00:51:59 Christina Johnson: Thanks! great presentation! 00:52:31 Ivonne Rodriguez Ramirez: Thanks! it was very interesting presentation 00:52:57 Rashmi Shivni: Really great work, Morgan! 00:53:05 Morgan: @Christina yes! definitely an easily adaptable set up to those systems 01:08:30 Christina Johnson: Thanks for sharing with us about Rhodium Scientific! 01:09:42 Kevin: You mentioned regolith simulant. How well do you think that simulates regolith for plant growth? 01:12:55 Morgan: Really cool stuff, thanks to everyone for sharing 01:13:07 Ivonne Rodriguez Ramirez: so cool! thanks!! 01:13:07 Christina Johnson: yay!