08:01:30 From Andrew Planet to Everyone:
	Hey there, nice to see you all!
08:01:50 From Patrick Grove to Everyone:
	Welcome!
08:01:55 From Colleen McLeod Garner to Everyone:
	Good Morning All from Californai!
08:01:58 From Andrew Planet to Everyone:
	Hey Patrick!
08:02:09 From Amir Mohammad Mokhtari to Everyone:
	Good morning everyone nice to see you
08:02:10 From Louise Fleischer to Everyone:
	Good afternoon from France!
08:02:20 From Hermes Hernan Bolivar-Torres to Everyone:
	Hello everyone👋
08:02:24 From Naiara Garcia to Everyone:
	Good morning everyone! Hi Chris! :)
08:02:26 From Andrew Planet to Everyone:
	Good afternoon from Bournemouth, Dorset UK
08:02:29 From Kara Rock to Everyone:
	Good morning from New Jersey, USA
08:02:29 From Christine Chamberlain to Everyone:
	Good morning everyone!
08:02:42 From Anna Polomska to Everyone:
	hello from Sheffield, UK! :)
08:02:42 From Colin Lennox to Everyone:
	good morning from firecamp!!  washington
08:02:45 From Lorrie Irwin to Everyone:
	Morning from Alaska . Thank you for the conference
08:02:45 From Patrick Grove to Everyone:
	Hey from Seattle!
08:02:47 From Gioia Massa to Everyone:
	Hello from rainy Florida!
08:02:50 From Christina Johnson to Everyone:
	Good morning from Cocoa Beach, FL
08:02:55 From Livian Von Dran to Hosts and panelists:
	Greetings from Colorado.
08:02:58 From Rosa Santomartino to Everyone:
	Hello everyone from Cornell (NY), glad to be here.
08:03:00 From Donald Coon to Everyone:
	Hello from Gainesville, FL!
08:03:00 From Andrew Planet to Everyone:
	It is fun to see where everyone is
08:03:01 From BHASKAR DEY to Everyone:
	good morning everyone,  I'm from India right now here evening so good evening everyone from my side
08:03:04 From Roland Meszter, PhD to Everyone:
	Hello from Lake Balaton, Hungary!
08:03:06 From Chris Okrainetz to Everyone:
	Good morning from Canada
08:03:07 From Dominic Locks to Everyone:
	Hello from Bavaria!
08:03:08 From David Evinshteyn to Everyone:
	Hello from Boulder!
08:03:13 From Jane Shevtsov to Hosts and panelists:
	Hello from Los Angeles!
08:03:20 From Brandon Saulnier to Everyone:
	Good morning from Montréal!
08:03:32 From Mia Schecter to Everyone:
	Hello from Louisville, KY!
08:03:36 From Sathesh Raj to Everyone:
	Hi from Malaysia 🇲🇾
08:03:36 From Olu Ojo to Everyone:
	Hello from Dallas, TX!
08:03:44 From Sanchita Saha to Everyone:
	Good morning. Hello from State College, PA!
08:03:52 From Gioia Massa to Everyone:
	We should do a map showing where participants are coming from - this is great!
08:04:10 From Frédéric Pitre to Everyone:
	Bonjour/Hi from Montreal!
08:04:43 From Andrew Planet to Everyone:
	Hey Frieda!
08:04:43 From Irena Li to Everyone:
	Hello from Los Angeles!
08:04:51 From Melinna Andrade to Everyone:
	Hi from New York!
08:04:58 From Melesa Ilhan to Everyone:
	from Istanbul, Türkiye
08:05:54 From Louise Fleischer to Everyone:
	I have the slides @patrick if you want
08:05:54 From Cesare Lobascio to Everyone:
	Ciao from Italy!!!
08:06:01 From Darlene Squibb to Everyone:
	Hello from Maryland
08:06:21 From Münire Gül to Everyone:
	Hi From İstanbul, Türkiye
08:06:23 From Quratulain Danish to Everyone:
	Salam/ Hello from Pakistan
08:07:58 From Louise Fleischer to Hosts and panelists:
	https://www.canva.com/design/DAGvax7u9d0/6YAxR0OS11xX1-zwPmZZMw/view?utm_content=DAGvax7u9d0&utm_campaign=designshare&utm_medium=link2&utm_source=uniquelinks&utlId=he58a9d957c
08:08:15 From Louise Fleischer to Hosts and panelists:
	This is a public link for the slides any other organizer should be able to present
08:08:33 From Colleen McLeod Garner to Everyone:
	Love to discuss collaboration: www.linkedin.com/in/colleenmcleodmba
08:08:38 From Candelaria Rodriguez to Everyone:
	hi, sorry but will the recording of this talk be upload to any social media or could be shared to us via email?
08:10:08 From Candelaria Rodriguez to Everyone:
	thank u so much!
08:10:23 From Andrew Planet to Everyone:
	Photopea.com is a nice alternative to Canva, equivalent to Adobe Photoshop, all you need is a browser, free
08:10:35 From Gioia Massa to Everyone:
	https://drive.google.com/file/d/1IbPC-3TeKJqBNDtrAq3ppVSDBDZ_Wb5F/view
08:11:14 From Gioia Massa to Everyone:
	Program
08:27:38 From Patrick Grove to Everyone:
	If you have questions, feel free to start dropping them in the Q&A box!
08:29:47 From Ales Nohel to Everyone:
	what about microgravity in terms of fluid flow and photosynthesis effects
08:33:31 From Rob Faltersack to Everyone:
	LEAF- What's the purpose of the legs? Does it have to be oriented upon landing or the interior orient itself? Why would it run out of power if you have a solar array? 
	
	I'd imagine you could build the solar array mechanism to unfold AND orient the craft independent from the interior. The interior could "float" with a weighted bottom....like a weeble people. :)   Restricted by the rectangular form-factor I realize...but it would be a shame for it to fail upon land and have to scrap the mission.
08:36:16 From Rob Faltersack to Everyone:
	I see thanks for explaining.
08:45:05 From Irena Li to Everyone:
	Is the crew supposed to deploy the experiment entirely on their own or will MOC be providing support during the payload interactions? How do they know if it’s been deployed correctly?
08:53:18 From Marshall Porterfield to Hosts and panelists:
	Where we go so do our problems... what are the most critical terrestrial gardening issues for the moon?
08:55:26 From Marshall Porterfield to Everyone:
	Jane... There are tons of issues and opportunities to explore regarding centrifuge and RPM controls in spaceflight experimentation...
09:00:58 From Marshall Porterfield to Everyone:
	Yay Chris!!!
09:05:15 From Christine Chamberlain to Everyone:
	@Irena: there will be a MOC (at JSC) communicating with the crew and supporting them during instrument deployments, although ideally those operations will be scripted and well practiced
09:09:32 From Patrick Grove to Everyone:
	"we are limited by not having an aquarium facility to study aquatic organisms [on orbit]" 
	
	Hear hear!
09:11:31 From Terry Trevino to Everyone:
	Scampi!! Tarun!!
09:12:11 From Patrick Grove to Everyone:
	SCAMPI Team 🦐🚀
09:12:46 From Patrick Grove to Everyone:
	for a little more detail on SCAMPI:
	https://thespringinstitute.com/scampi/
09:13:41 From Jane Shevtsov to Patrick Grove(direct message):
	Can we put Qs in Q&A?
09:14:21 From Patrick Grove to Jane Shevtsov(direct message):
	i kinda think zoom does not let panelists do that, unfortunately. throw it in general chat i suppose
09:16:03 From Marshall Porterfield to Hosts and panelists:
	I worked with CEBAS when I was in Germany during a post-doc project...
09:16:37 From Jane Shevtsov to Hosts and panelists:
	How does the protein production efficiency of fish compare to plant based systems, given that fish get their protein from their food?
09:18:11 From Frieda Taub to Patrick Grove(direct message):
	I can't get into the ? box; I'd like to make a brief comment and ask about salt vs freshwater systems?
09:18:58 From Patrick Grove to Frieda Taub(direct message):
	yeah, I think the hand raised is a good solution. Zoom does not let panelists ask questions in the QA box, only answer them.
09:20:20 From Rob Faltersack to Everyone:
	Oyster shells : future growth media for forests on the moon. Sequester CO2 and build tools and landmass like the Calusa had done in the everglades of Southern Florida.
09:21:00 From Rob Faltersack to Everyone:
	Ah...survivorship bias
09:22:55 From Colleen McLeod Garner to Everyone:
	Brilliant!  We need this!
09:23:27 From Anna Polomska to Everyone:
	amazing
09:25:41 From Andrew Planet to Everyone:
	Great that growing sessile molluscs is also very low resource and low carbon food therefore environmentally friendly here on Earth.
09:27:53 From Terry Trevino to Everyone:
	Can we put these tiny fish on Pizza!!?😁
09:28:26 From Patrick Grove to Everyone:
	All of the microalgae from photobioreactors and waste management systems has to go somewhere. oysters are a good candidate
09:28:44 From Andrew Planet to Everyone:
	Low carbon in terms of the energy needed to grow and put them on our plates I meant, not the carbon in its shells which is another resource
09:30:15 From Jane Shevtsov to Everyone:
	Radiation shielding: https://www.nasa.gov/general/water-walls-highly-reliable-and-massively-redundant-life-support-architecture/
09:30:20 From Rob Faltersack to Everyone:
	launch fresh water for ISS use and send salts seperately
09:30:56 From Harrison Coker to Everyone:
	The lunar simulates dissolve pretty quickly in water, lots of sodium too. Maybe not as much chloride but maybe the aquatic systems could be adapted to what readily comes from the regolith at each location
09:31:12 From Cesare Lobascio to Everyone:
	30 liters of water on ISS... well, I l 25 liters in the PERSEO radiation protection jacket :)
09:31:22 From Andrew Planet to Everyone:
	Great news on the abalone!
09:31:59 From Patrick Grove to Jacob Scoccimerra(direct message):
	thanks for the talk! excellent work! take a minute to respond to some questions in the QA box if you can
09:33:43 From Cesare Lobascio to Everyone:
	On PERSEO we had 24 liters of water filling the jacket, the safety panel requested to have it segregated in at least four different volumes
09:33:45 From A. C. to Everyone:
	Full spacesuits in a microgravity greenhouse?
09:35:20 From Rob Faltersack to Everyone:
	@A.C. shhh...let the astronauts bounce happily through the greenhouse :D
09:35:28 From Patrick Grove to Everyone:
	That's interesting perspective, Cesare!
	
	For our SCAMPI Mission to the ISS, safety panels required us to have 2 additional redundant layers of containment for 350ml of water 🙃
09:36:26 From A. C. to Everyone:
	Mitochondria don't have telomeres they have circular genomes!
09:36:34 From Marshall Porterfield to Everyone:
	As a preview for the panel discussion tomorrow I want to share a pre-print of our new paper on bioregenerative life support programs and policy. The history of funding and support has been discontinuous and many research programs have been deferred by NASA in order fund other priorities. This is covered by this new paper, and compared to the recent progress by the CNSA.
09:36:42 From Cesare Lobascio to Everyone:
	However, in the case of PERSEO, water was returned to the water mgmt system after the experiment, as wastewater
09:37:22 From Cesare Lobascio to Everyone:
	Also, the 4 volumes had to be separate and with manual valves for isolation in case of leakage
09:38:47 From Marshall Porterfield to Everyone:
	No telomeres in mitochondria or chloroplasts...
09:39:39 From Marshall Porterfield to Everyone:
	Please preview this new paper for the panel discussion tomorrow.
09:40:02 From Patrick Grove to Everyone:
	👍 thanks Marshall!
09:41:33 From Marshall Porterfield to Everyone:
	RPM does not mimic microgravity. this is a biophysically impossible
09:42:28 From Marshall Porterfield to Everyone:
	There should be a 1g centrifuge on the ISS... that is the ultimate control for radiation vs microgravity
09:47:12 From Terry Trevino to Everyone:
	"Technology and Developments for the Random
	Positioning Machine, RPM"  Borst et al, - shows RPMs do provide vector change and gravitational off loading
09:48:13 From A. C. to Everyone:
	What if instead of telomerase over expression just TERT overexpression is tested for anti  ROS effects.
09:48:26 From Jane Shevtsov to Everyone:
	Wow!
09:50:00 From Frieda Taub to Everyone:
	I see the potential for other researchers exchanging samples between labs--e.g. those stressing any organism, could send Barbero's lab to conduct telomere measurements... thus make discoveries that a specific lab doesn't have that analytical capability.
09:50:32 From Marshall Porterfield to Everyone:
	There are currently no plans to grow anything in regolith any time soon. The first phases of lunar agriculture will be done with solid state hydroponics...
09:51:56 From Harrison Coker to Everyone:
	Regolith-based agriculture is an absolute necessity for longer-duration outputs. Resource constraints. Needs research sooner than later?
09:54:45 From Rob Faltersack to Hosts and panelists:
	Would  myco-remediation remove the copper/alum before the next generation germination?
09:55:22 From Terry Trevino to Everyone:
	I have loads of testing in both MGS-1 and Lunar from Space Resources. And the plants are definitely taking up the heavier metals. I have results and will release those at IAC in October. Analysis done by New AGe Labs
09:56:20 From Donald Coon to Everyone:
	Ah excellent, that is what I was wondering about. Regolith becomes less harsh as it is reused. Glad to see the impact to telomeres and genomes is also reduced!
09:56:24 From Jane Shevtsov to Everyone:
	Phytoremediation, in effect
09:57:29 From Rosa Santomartino to Everyone:
	That's really an amazing and complete body of work, Borja! Every question I had, you replied in the following slides :D  Excellently done.
09:57:33 From Donald Coon to Everyone:
	Gotta love phytoremediation.
09:58:44 From Andrew Planet to Everyone:
	Excellent talk indeed Borja!
09:59:13 From Anna Polomska to Everyone:
	some time ago I was reading about using mosses for regolith bio-conditioning - maybe that's an idea?
10:01:33 From Terry Trevino to Everyone:
	We have been using Algae to bioremediate!
10:01:57 From A. C. to Everyone:
	Regolith EDTA pretreatment?
10:02:26 From Jane Shevtsov to Patrick Grove(direct message):
	I have to step away and will return!
10:04:39 From Rob Faltersack to Everyone:
	Looks like breakout rooms do not appear on the browser based Zoom (chrome, mac os) I'll try to get the native app over the break.
10:11:15 From Aleena Durrani to Everyone:
	Hello everyone, I am from Pakistan and i am working as a research assistant at Precision medicine lab. My research is focused on Space biology, specifically muscle atrophy
10:12:01 From Susan Jewell to Everyone:
	drjewellmd@gmail.com
10:12:10 From Ales Nohel to Everyone:
	can't see the ln
10:13:08 From Patrick Grove to Everyone:
	Thanks for the heads up Rob, I wasn't aware that they don't work on the browser version!
10:33:14 From Frieda Taub to Hosts and panelists:
	Nate O'Neil ... I can't see your chat link. My email is taub@uw.edu
10:33:44 From Patrick Grove to Hosts and panelists:
	Frieda, heads up - you sent that to hosts and panelists rather than to Everyone or directly to Nate.
10:34:11 From Patrick Grove to Hosts and panelists:
	Have to click on the blue button in the To: field above the chatbox to change it
10:34:27 From Marshall Porterfield to Hosts and panelists:
	For hydroponic based plant culture in a BlSS the estimates are that it will "cost" 94kg of mineral salts per year for each person...
10:35:23 From Marshall Porterfield to Hosts and panelists:
	We need to recycle the minerals, but also to re-gasify co2...
10:35:51 From Ales Nohel to Everyone:
	since we have programmable biome to create protein from H2O CO2 O2 +E(ph)
10:36:15 From Ales Nohel to Everyone:
	why bother with the plant animal cycle
10:36:37 From Patrick Grove to Everyone:
	because they taste good :)
10:37:07 From Ales Nohel to Everyone:
	not too ethical, at least say thanks for their life 😜
10:37:19 From Marshall Porterfield to Hosts and panelists:
	Most terrestrial soils are depleted of magnesium, and most humans are Mg deficient, which is a mitochondrial stress element  that can increase cancer risks...
10:37:24 From Ales Nohel to Everyone:
	just trying to simplify the loop
10:37:47 From Ales Nohel to Everyone:
	that's why we use bacteria, very efficient
10:38:02 From Ales Nohel to Everyone:
	highly hackable
10:38:17 From Marshall Porterfield to Hosts and panelists:
	cell culture methods are not green or bioregenerative...
10:38:58 From Ales Nohel to Everyone:
	engineer a biological antagonist
10:39:40 From Patrick Grove to Hosts and panelists:
	FYI Marshall you've been sending messages to the panelists rather than the whole audience
10:43:20 From Rob Faltersack to Everyone:
	@Ales - have you checked out Solar Foods?
10:44:45 From Ales Nohel to Everyone:
	yeah these are +TRL ideas that I did at NASA apps hackathon 2015, algae to create food for stem cells to create food
10:45:19 From Ales Nohel to Everyone:
	there's a few of on the cusp teams
10:49:39 From Gioia Massa to Everyone:
	Interesting recent popular sci article: https://www.popsci.com/health/artificial-food-origins/
10:50:07 From Marshall Porterfield to Hosts and panelists:
	My former student Eric McLamore is one of the leading expert in P measurement in ag/ecosystems...
10:51:38 From A. C. to Everyone:
	Phosphorous is only an aquatic pollutant if the alga that uses it is not harvested and its biomass recycled into compost.
10:52:05 From Gioia Massa to Everyone:
	Fascinating work Chip!
10:53:31 From Jane Shevtsov to Hosts and panelists:
	This is why I love soil-based agriculture!
10:53:43 From Rob Faltersack to Everyone:
	So you need the storage of surplus as buffers. Eb and flow
10:53:57 From Patrick Grove to Everyone:
	soil as a buffer, regolith as a soil!
10:55:08 From Jane Shevtsov to Hosts and panelists:
	Marshall, let's talk about this. I know analysis methods that could be useful.
10:55:42 From Rob Faltersack to Everyone:
	Systems models!   And analogs!
10:56:43 From A. C. to Everyone:
	No work on the necessity of the beneficial effects of mycelium in soil for healthy crops!
10:56:50 From Andrew Planet to Everyone:
	Do we have any talks that deal with precision fermentation in space ecology, a technology that uses microorganisms like yeast or bacteria to produce specific, desirable molecules, including proteins, enzymes, and other food ingredients?
10:57:56 From Kai Staats to Everyone:
	Thank you everyone for use SIMOC. I am honored.
10:58:08 From Kai Staats to Everyone:
	I have to jump to another call … back in an hour.
10:58:10 From Patrick Grove to Everyone:
	Not in this workshop, but Harrison Coker could talk about it! He just sent a beer brewing experiment to the ISS!
10:59:45 From Marshall Porterfield to Everyone:
	Kai Yes Simoc is my favorite computer game!!
11:00:55 From Marshall Porterfield to Everyone:
	Cell bioreactor system performance is something I can discuss if interested..
11:01:20 From Andrew Planet to Everyone:
	Yes please
11:01:47 From Andrew Planet to Everyone:
	Thanks Patrick
11:04:48 From Patrick Grove to Everyone:
	"The Marshian" is a pun, by the way, not a typo 😁
11:04:51 From Rachel Rivero to Everyone:
	https://starbasebrewery.com/
11:05:04 From Rob Faltersack to Everyone:
	😄
11:05:05 From Rachel Rivero to Everyone:
	Starbase Brewing | Texas Craft Beer | Official Brewery of Mars
11:05:12 From Chip Small to Hosts and panelists:
	Thanks for all of the great comments and questions. Happy to follow up with anyone—my email is gaston.small@stthomas.edu
11:05:49 From Andrew Planet to Everyone:
	Thanks Rachel, interesting, imagine fermenting proteins in space too.
11:06:49 From Rachel Rivero to Everyone:
	Harrison will talk more about it 😉
11:07:33 From Andrew Planet to Everyone:
	Creating them thus I mean, even from wastes. Thanks, looking forward to Harrison's talk!
11:30:05 From A. C. to Everyone:
	Effluent for alga/ yeast/growth and eventual harvesting for SCP production.
11:31:05 From Andrew Planet to Everyone:
	Yes
11:31:51 From Rosa Santomartino to Everyone:
	You'll do great Livian, we have all been there :)
11:32:31 From Frieda Taub to Hosts and panelists:
	I need to change location during the "lunch" and discussion period. I'll be back before 1 pm. Sorry to miss some of the discussion. Frieda B. Taub
11:32:45 From Andrew Planet to Everyone:
	Thanks for the SCP term
11:32:53 From Patrick Grove to Hosts and panelists:
	No problem!
11:33:10 From A. C. to Everyone:
	Single Cell Protein.
11:33:42 From Andrew Planet to Everyone:
	Looked it up, thanks A C
11:43:18 From Anna Polomska to Everyone:
	yes
11:45:01 From Rob Faltersack to Everyone:
	YEEHAW!
11:45:19 From Patrick Grove to Everyone:
	😅
11:46:20 From Frieda Taub to Hosts and panelists:
	Thank you Livian, for the review. I had been working on a potential book reviewing the early work on Closed Systems. The draft was negatively reviewed (all the research was a "waste of time") in the view of the reviewers. I dropped effort on it, but maybe Livian and others may be interested. I think the computer files may still be readable??? Frieda B. Taub taub@uw.edu.
11:49:55 From Jane Shevtsov to Hosts and panelists:
	Yes, absolutely!
11:50:00 From Cesare Lobascio to Everyone:
	Very instructive, Livian, thanks!
11:51:30 From Jane Shevtsov to Everyone:
	Look at the TOC from the 1971 edition of Eugene Odum's ecology textbook. https://www-archiv.fdm.uni-hamburg.de/b-online/snowbird/prefaces/odum.htm
11:54:43 From Rob Faltersack to Everyone:
	I’d be interested to so where BLiSS ranks.
11:56:20 From A. C. to Everyone:
	If anyone ever wants to read an early ~1957 short SF story involving the trials of closed loop system hurdles look up "The Cage" by A. Bertram Chandler, a short story first published in Fantastic Universe. A possible fortelling of future pitfalls in these systems.
11:57:12 From Patrick Grove to Everyone:
	Very nice graph Livian! It tells a cool story
11:59:41 From A. C. to Everyone:
	Super job presenting, excellent.
11:59:44 From Rob Faltersack to Everyone:
	Thanks Livian, this was a fun presentation and highlights that education and communication is important for funding
12:00:21 From Bryce Meyer to Hosts and panelists:
	Dinner came from space now :0)
12:01:06 From Bryce Meyer to Hosts and panelists:
	Farming on Earth in the US et al is VERY dependent on space research and data
12:01:35 From Bryce Meyer to Hosts and panelists:
	likely around 20%-30% or more in production due to sape work
12:01:39 From Bryce Meyer to Hosts and panelists:
	space work
12:01:50 From Rob Faltersack to Everyone:
	I’d be interested in reading your book Frieda, if possible.
12:01:57 From Bryce Meyer to Hosts and panelists:
	I got a chart somewhere on that
12:02:39 From Rosa Santomartino to Everyone:
	Great point, Freida. On the topic of the potential terrestrial benefits of this kind of research, if I may, I'd like to share one of our recent perspective papers, which touches on this aspect toward the end of the article: https://pubmed.ncbi.nlm.nih.gov/36944638/
12:02:53 From Rosa Santomartino to Everyone:
	There is also a similar paper from Berliner et al., let me search for it
12:05:14 From Bryce Meyer to Hosts and panelists:
	is this the book: "Space Resources and Space Settlements - Softcover
	NASA"
12:05:44 From Cesare Lobascio to Everyone:
	dinner time here :)
12:06:10 From Rob Faltersack to Everyone:
	10pm here….almost zzzzz time
12:06:59 From Bryce Meyer to Hosts and panelists:
	mid Afternoon in MO farm country
12:59:53 From Livian Von Dran to Hosts and panelists:
	I can't see the open questions, unfortunately.
13:00:09 From Sharon Doty to Everyone:
	Hi Patrick
13:00:54 From Lorrie Irwin to Hosts and panelists:
	Hi Patrick great speakers
13:02:39 From Patrick Grove to Hosts and panelists:
	Livian - follow up with Rosa Santomartino who wants to talk.
13:03:04 From Sharon Doty to Hosts and panelists:
	the view we see is not the presentation mode
13:03:34 From Naiara Garcia to Everyone:
	presentation mode is not what is being shared, I think she needs to choose the slides screen and not the power point file screen
13:03:37 From Rosa Santomartino to Everyone:
	I do not see the presentation though, I think you might want to click the presentation mode
13:03:53 From Rosa Santomartino to Everyone:
	Exactly :)
13:04:02 From Gioia Massa to Everyone:
	Try the book view
13:04:30 From Anna Polomska to Everyone:
	maybe she's having a presentation on a second screen that is not shared?
13:04:35 From Anna Polomska to Everyone:
	yes :)
13:04:39 From Rosa Santomartino to Everyone:
	Yes
13:04:54 From Andrew Planet to Everyone:
	What about clicking on slide show above, it would have changed anyway to a full show
13:05:40 From Sharon Doty to Hosts and panelists:
	usually need to start the powerpoint first, then share the window
13:06:07 From Gioia Massa to Everyone:
	Reading mode - right next to the slide show.
13:06:42 From Rosa Santomartino to Everyone:
	yes!
13:24:09 From Marshall Porterfield to Hosts and panelists:
	How do we know that genetic engineering is the best strategy for countermeasures vs hardware upgrades where physical systems are easier to engineer?
13:30:08 From Frieda Taub to Hosts and panelists:
	A great gift of the Space program will be converting human wastes, garbage, etc. to fertilizers (and other useful products) and thereby decrease our pollution of rives, lakes, the ocean.
13:30:52 From Rosa Santomartino to Everyone:
	@Lisa thank you for this amazing overview. This is all highly relevant for my work, which focuses on harnessing microbes for ISRU and synthetic waste recycling. I would love to connect :)
13:31:08 From Bryce Meyer to Everyone:
	That is Gerard ONiel habitat from the NASA Summer Study...
13:31:54 From Andrew Planet to Everyone:
	Love the imagery and concept, thanks Lisa!
13:34:10 From Anna Polomska to Everyone:
	it's getting late so I will see you all tomorrow! great sessions so far, lots of inspiring talks and useful information for my own research - many thanks & see you tomorrow!
13:34:21 From Patrick Grove to Everyone:
	Thanks Anna, good night!
13:34:44 From Marshall Porterfield to Hosts and panelists:
	Thank you LISA for a great talk!!
13:36:20 From Bryce Meyer to Hosts and panelists:
	Looking forward to seeing a whole plant grow up on moon on LEAF...that is a big deal to be sure!
13:40:30 From Kai Staats to Everyone:
	I fear that the Mars One effort of a decade ago has been conflated with current, more realistic efforts. I am not familiar with anyone speaking of a staying on Mars for a life-long mission at any time in the near future (100 years). That kind of “mission” is far, far beyond anything we are ready for, now.
13:43:57 From Rob Faltersack to Everyone:
	The Green Deal
13:44:20 From Kevin Chaz Shaffman to Everyone:
	can you drop that pre-print link again
13:44:35 From Sharon Doty to Hosts and panelists:
	yes, please since the previous chat went away
13:44:39 From Rob Faltersack to Everyone:
	Any analog
13:45:46 From Andrew Planet to Everyone:
	Nice info from your link Lisa, some are 404 already though https://qrco.de/bfkYHV
13:46:45 From Shriya Musuku to Everyone:
	Hi Lisa, Thank you! some of the private analog crews are organizing journals. I believe more data will become publicly available soon. the AARJ is a good follow, as they've just gotten off the ground!
13:48:50 From Frieda Taub to Hosts and panelists:
	I recall, vaguely, a newspaper article on how the $$$ spent by NASA helped local communities, how educational opportunities increased, poverty reduced (because well funded NASA employees paid taxes). We need more of this kind of covered. Money is not thrown into space; it is spent on earth in the communities near its facilities.
13:49:24 From Lisa Carnell to Everyone:
	Thank you for the invite to speak today. My email: lisa.a.scottcarnell@nasa.gov
13:50:13 From Rosa Santomartino to Everyone:
	Thank you, Lisa. Will definitely be in touch!
14:13:36 From Marshall Porterfield to Hosts and panelists:
	they have to fly N3 up to the iss all the time
14:14:45 From Cesare Lobascio to Everyone:
	most air & N2 losses are due to EVA... airlocks
14:14:51 From Kai Staats to Everyone:
	Nitrogen is cannot be obtained from electrolysis as it is not held in water molecules. Nitrogen is inert, meaning it does not react with anything, so it is breathed in and out without loss. The loss is via the continuous leaks which loses all molecules.
14:15:37 From Frieda Taub to Hosts and panelists:
	Bryce, and others. Thank you Bryce for your examples of local farming that benefits from satellites, weather, … GPS... space data on crop increases. But I'm not certain the general public isn't thinking the money is spent (physically? in space..."throwing money away in space when there are so many problems on earth" Frieda
14:16:07 From Shriya Musuku to Everyone:
	Kai I appreciate the correction here and apologies for my misunderstanding!
14:16:26 From Cesare Lobascio to Everyone:
	we build pressurized modules, the trick is to have great welding and seals
14:16:33 From Gioia Massa to Everyone:
	We must be atom efficient!
14:16:41 From Kai Staats to Everyone:
	No worries! Your talk was great! Your enthusiasm and technical command are outstanding.
14:17:13 From Bryce Meyer to Hosts and panelists:
	Loved the talk, and all them so far :))
14:17:43 From Patrick Grove to Everyone:
	supposedly Biosphere 2 had a very, very tiny leakage rate - partially good engineering on the seams, but also the lung systems which helped maintain a stable pressure even as the atmosphere expanded and shrank due to daily temperature change.
14:18:03 From Shriya Musuku to Everyone:
	Appreciate it! This team is awesome. Thanks all, I appreciated the discussion and additions to this research project
14:18:59 From Kai Staats to Everyone:
	Another consideration: nitrogen is essential for all plant growth and therefore must be shipped to the Moon and Mars. This will be very, very expensive and something that is not talked about enough. Without N2, we simply don’t grow plants.
14:21:18 From Jane Shevtsov to Everyone:
	This is why I'm obssessed with nitrogen. We have to think about how it cycles in a whole BLSS system and how to manage flows to miinimize the need for large stocks.
14:21:28 From Bryce Meyer to Everyone:
	Yup, but I would argue you need both N2 as inert atmospheric gas, and nitrogen rich solids for fertilizer, in addition to a year of protein rich food for humans which will result in urea...?
14:21:40 From Bryce Meyer to Everyone:
	so all 3'
14:22:48 From Patrick Grove to Everyone:
	and, to make things worse, nitrogen is not very evenly distributed in the solar system. there's very little in martian soil, it's mostly in atmospheres of gas giants and their moons or in comets. hard to access off-earth!
14:23:13 From Rob Faltersack to Everyone:
	Harvest and store nodules, you can process it later to gas.
14:23:49 From Jane Shevtsov to Everyone:
	Nodules?
14:24:08 From Bryce Meyer to Everyone:
	I think he means like for legumes
14:24:11 From Frieda Taub to Hosts and panelists:
	Kai, NO3-, NO2-, NH3 (NH4+) can be stored as dissolved or dry chemicals, but N2 is not available to algal or higher plant growth...so nitrogen fixation may be critical...but if our atmosphere for humans is 79.1% N2, we need to conserve or store N2.
14:24:30 From Bryce Meyer to Everyone:
	yup
14:26:10 From Jane Shevtsov to Everyone:
	I think we should aim to keep fixed N in bioavailable forms. The less energy we or plants have to allocate to N fixation, the better.
14:28:09 From Patrick Grove to Everyone:
	yeah, not to mention a constantly changing atmospheric composition would be annoying to manage. imagine oxygen being crowded out by fixing too much nitrogen
14:28:22 From Shriya Musuku to Everyone:
	Very interesting! So to dig a little further here, if not by electrolysis, how is N2 made? It was my understanding that some of the diatomic gases were extracted as such until Kai's note.
14:29:13 From Patrick Grove to Everyone:
	N2 is the result of several successive bacterial transformations of organic waste (Nh4 -> NO2 -> NO3 -> N2)
14:30:47 From Shriya Musuku to Everyone:
	Ah, that makes sense, thank you!
14:32:03 From Patrick Grove to Everyone:
	no worries, nitrogen is a weird one
14:32:43 From Kai Staats to Everyone:
	Yes, but you use have N2 to start. It can’t be generated from thin (or thick) air 🙂 If we can learn to extract N2 through transformation of inedible biomass and back into our food production systems, then in theory, we bring N2 to the Moon and Mars once, then recycle, But entropy is the master of the universe, and nothing is 100% recycled.
14:34:04 From Cesare Lobascio to Everyone:
	What you obtain with water electrolysis is O2 and H2
14:35:16 From Kai Staats to Everyone:
	Outstanding presentation! So much information.
14:35:41 From Kai Staats to Everyone:
	I want to hear it 2 more times to absorb it all 🙂
14:35:47 From Shriya Musuku to Everyone:
	Awesome! Very exciting work!
14:36:02 From Gioia Massa to Hosts and panelists:
	It is so great to hear your latest results Donald! Exciting.
14:36:15 From Jane Shevtsov to Hosts and panelists:
	@Kai It will be on YouTube!
14:37:46 From Frieda Taub to Hosts and panelists:
	In most discussions of N plant fertilization have processes that "useable" forms (for plant growth, nitrate and ammonia)  are lost as N2, denitrification processes...I'd have to review the literature. Most books should have descriptions of N2 cycles. (This info is not in my head.)
14:39:20 From Patrick Grove to Everyone:
	Tomorrow, Luke Fountain will be presenting a talk: Plants prefer ammoniums as a N source: implications for space crop production". Be sure to attend and bring these Nitrogen questions!
14:39:39 From Phil Sadler to Cesare Lobascio, Hosts and panelists:
	Cesare-There is unlimited cooling potential 30cm below the habitat, you saw my Thermal Well in the past.
14:39:52 From Gioia Massa to Everyone:
	So great to see you latest results Donald!  Exciting work!
14:41:32 From Shriya Musuku to Everyone:
	Thanks for the information Patrick! I'm loving the discussions here. So many new learnings for my engineering brain 😃
14:42:19 From Andrew Planet to Everyone:
	For the previous presentation, but thanks
14:42:48 From Jane Shevtsov to Hosts and panelists:
	Under carefully controlled conditions of temperature, humidity and pressure, the organism will do whatever the hell it wants.
14:43:35 From Bryce Meyer to Everyone:
	yup.  and I like real food, not glop
14:43:40 From Bryce Meyer to Everyone:
	:0)
14:44:35 From Bryce Meyer to Hosts and panelists:
	The energy cascade is a darn interesting way to calculate the loop!
14:44:37 From Andrew Planet to Everyone:
	I reckon we could combine both artificial and purely biological photosynthesis for oxygen, the latter as a back up in case if the tech breaks down. Especially needed due to the environment of evolutionary adaptiveness (EEA) for health and aesthetics
14:44:45 From Bryce Meyer to Hosts and panelists:
	Great preso
14:44:53 From Shriya Musuku to Everyone:
	Ah Andrew - it's an interesting question. It is focused on energy and bioreactors to my knowledge. At least at ESA. Definitely do not have as much knowledge on plants - Donald is the expert there!
14:44:59 From Kai Staats to Everyone:
	Vapor Pressure Deficit, the ratio of temp vs relative humidity. Plants transpire a different rates based on the VPD ratio.
14:45:17 From Jane Shevtsov to Hosts and panelists:
	Are there any available R or Python libraries for simulating the energy cascade?
14:45:29 From Andrew Planet to Everyone:
	Thanks Shriya!!!
14:45:36 From Rosa Santomartino to Everyone:
	Will need now unfortunately, it's getting quite late here. Thank you very much for this exciting day.
14:45:54 From Rosa Santomartino to Everyone:
	*to go
14:48:34 From Donald Coon to Hosts and panelists:
	@ Jane, there are no existing libraries for them other than what I have written for this work. That being said, It is my plan to publish a GitHub with all 4 by the end of the year, but I'm open to sharing them beforehand with interested people
14:50:05 From Shriya Musuku to Everyone:
	Also need to drop, but thank you for the amazing workshop everyone. I look forward to watching the replay of any I missed!
14:52:20 From Jane Shevtsov to Hosts and panelists:
	@Donald That would be great! (I mean boh.)
14:52:53 From Bryce Meyer to Hosts and panelists:
	The energy cascade stuff could be done in MATLAB/Simulink then use that to kick out code?
14:53:37 From Bryce Meyer to Hosts and panelists:
	Then you could manipulate to processes easier I think...just a thought
14:56:05 From Jane Shevtsov to Hosts and panelists:
	If parameters aren't hard-coded, manipulating stuff with equation-based models should be fine. Simulink is commercial, so not everyone can use it. Python and R are more accessible and widely used in biology.
14:56:34 From Bryce Meyer to Hosts and panelists:
	True
14:57:04 From Bryce Meyer to Hosts and panelists:
	Personally, I like to code, but just offering an option
14:58:32 From Donald Coon to Hosts and panelists:
	I'll keep that MATLAB idea in mind as I move to reworking them. I've gotten very handy with loops. But yeah, the choice for python is to free it from proprietary software. Jane, I'll reach out about sharing the scripts with you.
14:58:50 From Bryce Meyer to Hosts and panelists:
	I used to write bio code in BASIC, FORTRAN, and PERL back in the day, but I am dating myself LOL
15:00:07 From Donald Coon to Hosts and panelists:
	Nice. I've been tempted to rewrite them in fortran so it lasts forever lol
15:00:28 From Andrew Planet to Everyone:
	Great info Frieda and Jane, thanks for sharing!!!
15:00:34 From Harrison Coker to Hosts and panelists:
	I am here!
15:01:43 From Jane Shevtsov to Hosts and panelists:
	A 100% raise... from 0.
15:01:57 From Patrick Grove to Hosts and panelists:
	😆
15:26:14 From Rachel Rivero to Everyone:
	I’ll put this here again for Harrison: https://starbasebrewery.com
15:26:29 From Rob Faltersack to Everyone:
	Nice work. Microbrew or Moonshine? 😄
15:29:14 From Frieda Taub to Hosts and panelists:
	Could we use the perchlorate as an bacterial contaminant control?  But then how do you get rid of the perchlorate..to use the water or gas for another use?
15:31:02 From Borja Barbero Barcenilla to Everyone:
	Great job Harrison!!!
15:32:38 From Jane Shevtsov to Hosts and panelists:
	Awesome talk, Harrison! You might look at David Sloan Wilson's work on community-level selection.
15:33:44 From Jane Shevtsov to Hosts and panelists:
	I wonder about the role of organic acids in the regolith weathering.
15:36:21 From Harrison Coker to Everyone:
	Great older paper from Ming/Sparks labs on organic acid facilitated dissolution of lunar regolith: https://www.sciencedirect.com/science/article/abs/pii/0016703795003770?via%3Dihub
15:36:44 From Harrison Coker to Rob Faltersack, Hosts and panelists:
	I hope moonshine 😂
15:47:14 From Rob Faltersack to Hosts and panelists:
	Bryce, I’m glad you presented this type of situational awareness + guidance + risk assessment and actuation. I was considering to present this same content at this years workshop.  I’d like to continue this discussion and build out a working prototype for daily operation and disaster recovery.
15:52:03 From Borja Barbero Barcenilla to Everyone:
	Bryce mentioned Beer. Time to open one ;)
15:52:37 From Patrick Grove to Everyone:
	the official drinking game of the space ecology workshop 🍻
15:53:02 From Borja Barbero Barcenilla to Patrick Grove, Hosts and panelists:
	Harrison did as well! 😂
15:53:02 From Borja Barbero Barcenilla to Patrick Grove, Hosts and panelists:
	Harrison did as well! 😂
15:55:14 From Borja Barbero Barcenilla to Patrick Grove, Hosts and panelists:
	Fantastic talk and great day. Amazing job Patrick!
15:55:14 From Borja Barbero Barcenilla to Patrick Grove, Hosts and panelists:
	Fantastic talk and great day. Amazing job Patrick!
15:55:29 From Patrick Grove to Hosts and panelists:
	Thanks :)
15:56:35 From Rob Faltersack to Everyone:
	That is covered in preventative maintenance approaches today. A trend in vibration or temperature.
15:58:05 From Rob Faltersack to Everyone:
	Frieda, we’ll get the hyper localized guidance from the marketing and advertising industry. They know you better that anyone.
15:58:20 From Donald Coon to Everyone:
	I have always envisioned the final product of the EC models as tool that an AI would use to send alerts based on plant physiology performance to avoid needing an expert watching predictions and sensor logs constantly. Love to see this kind of thought being used.
15:58:55 From Patrick Grove to Everyone:
	Totally!
15:59:03 From Andrew Planet to Everyone:
	Using the Kentucky Fried order screen for the first time yesterday to self select my order felt like a computer that understood how much I know about computers Frieda. It was very easy to use
15:59:19 From Bryce Meyer to Everyone:
	bryce.meyer@nss.org
16:01:07 From Andrew Planet to Everyone:
	Very high quality information coming in all day, thanks!
16:01:35 From Naiara Garcia to Everyone:
	Thank you all! Great presentations!
16:01:40 From Rob Faltersack to Everyone:
	Thanks everyone!
16:01:43 From Cesare Lobascio to Everyone:
	snooze time here :)
16:01:52 From Ivan Nocua to Everyone:
	Thanks everyone :)
16:01:53 From Andrew Planet to Everyone:
	See you tomorrow!
16:01:59 From Patrick Grove to Everyone:
	i cant believe you're still awake Cesare! good night!
16:02:03 From Lorrie Irwin to Hosts and panelists:
	Thank you Patrick